Congrats to the Lugo lab, whose abstract entitled “Neuron subset-specific-PTEN deletion induces abnormal skeletal activity in mice” received a podium presentation at the upcoming 2014 American Epilepsy Society Meeting in Seattle. The abstract reflects a collaborative project with Joaquin Lugo’s lab at Baylor University in which we are working understanding a potential link between cortical brain dysfunction (such that which arises during epilepsy) and impaired skeletal activity.
Ron will be giving a talk on 5/27/15 (3:30pm, MEB 238) for the UW Biomechanics Seminar.
Great news, our paper entitled “Botulinum toxin induces muscle paralysis and inhibits bone regeneration in zebrafish” was just published online in the Journal of Bone and Mineral Research. Congrats to Anthony and Amanda as well as all the other authors!
Congrats to Adrian who was accepted to the Washington NASA Space Grant Consortium Summer Undergraduate Research Program. He will be working on developing chemical screening tools in the zebrafish skeleton.
Ron will be giving at talk at the 7th World Congress of Biomechanics entitled “Neuro-Musculoskeletal Systems Biology in Zebrafish”. The talk will be part of the “Mechanobiology of Bone” session on Jul 6, 2014, 2:30 PM – 4:00 PM.
Congrats to Marjorie and Anthony, who will be presenting their research at the 2014 UW Undergraduate Research Symposium. Marjorie will be giving a talk for her abstract “Brain-specific PTEN deletion induces epilepsy and abnormal bone formation in mice” in Session 2F: Brain Development, Injury, Regeneration and Restoration of Function (242 MGH, 3:30 PM to 5:00 PM). Anthony will be presenting his poster “Neuronal regulation of regenerative bone growth in the zebrafish fin” in Poster Session 4 (Commons East, Easel #48, 4:00 PM to 6:00 PM).
Ron will be giving a talk with Drs. Chris Allan, Stephen Kennedy, and Laura Stoll entitled, “Finger Replantation: When, Where, and What to Expect”. See below for details.
What: Orthopaedics and Sports Medicine Grand Rounds
When: Wednesday, May 7, 2014 – 6:45- 7:45 AM
Where: Genome Sciences Building, Foege Auditorium, Room S060
Congrats to Amanda! Her abstract was one of ten selected for a podium presentation for the 2014 GME Research Day, a symposium that highlights the best of resident and fellow research across the University of Washington. The symposium is May 3, 7:30 am to 12:00 pm, at the Orin Smith Auditorium at South Lake Union.
Ron will be giving a talk on 02/12/14 for “Research Exposed!”. This talk is open to the public for those who are interested.
Wanted to share this entertaining and informative talk for Science Cafe by MSBL collaborator, Werner Kaminsky. When he not helping us find new ways to use polarized light to analyze zebrafish fin rays, he is making new discoveries about the crystal structure of beer molecules (which has really important health implications). Check it out!
Great news! The MSBL was awarded a UW Royalty Research Fund Grant for the project entitled, “Osteoactive Compound Screening in the Regenerating Zebrafish Fin”. This project will seek to advance the regenerating zebrafish fin as a novel preclinical system for rapid discovery of compounds that modulate bone growth and mineralization.
Congratulations to Anthony Recidoro! Anthony was awarded the prestigious (and highly competitive) Mary Gates Undergraduate Research Scholarship for his application entitled “Neuromuscular Regulation of Bone in the Regenerating Zebrafish Fin” today. Way to go Anthony!
Congrats to Amanda! Her abstract was selected for a podium presentation at the 60th Annual Meeting of the Orthopaedic Research Society (March 15-18 in New Orleans). Details of her abstract are listed below:
Paper #: 154
Title: Chemical and Mechanical Nerve Impairment Inhibit Joint Specification and Bone Formation in the Regenerating Zebrafish Fin
Authors: Amanda C. Roof, M.D., Anthony Recidoro, Brandon J. Ausk, M.S., Sundar Srinivasan, Ph.D., Ted Gross, Ph.D., Edith M. Gardiner, Ph.D., Steven D. Bain, Ph.D., Christopher Allan, M.D., Ronald Kwon, Ph.D..
Ron will be giving a talk for the Solid Mechanics Seminar Series at Brown University on 12/9/13. Thanks to Haneesh Kesari for the invite.
Good news, Introduction to Cell Mechanics and Mechanobiology was just rated a 90 (4-star) on Doody’s Publishers’ Club. An excerpt of the review is copied below:
“(The book) offers excellent groundwork in introducing many topics ranging from fluid or solid to statistical or computational mechanics and their importance for cell and tissue behavior…Bridging many disciplines, which at first seem unrelated, opens new perspectives and novel applications for topics conventionally covered in more traditional courses in either the engineering or biology fields.”
Had a fun time giving a webinar for Eli Lilly this morning. The title of the talk was “Musculoskeletal discovery in zebrafish through systems biology”. Thanks to everyone who attended and to Nicoletta Bivi for inviting me to give the webinar.
Great news! Anthony was awarded an Undergraduate Travel Award from the University of Washington. He will use this award to present his abstract at the 2013 ASBMR conference in Baltimore. Congrats man!
Congrats to Leah1, Brandon, Leah2, Edith, Sundar, Steve and Ted! Our paper entitled, “Systems-based analysis of temporal processing pathways during bone cell mechanotransduction” was published in PLoS ONE today. Click here for the article.
Had a really great time at the 2013 Sun Valley Workshop. I would tell you about how fantastic it was, but instead you can just click here to read about highlights from the conference, including a small paragraph I provided the IBMS describing my experience. You can also click here to see some pics that people took, including a group shot from our hike to Pioneer Cabin.
Trick or treat! Ron will be giving a talk at Tulane University for the Department of Biomedical Engineering’s Seminar Series on Halloween 2013 (10/31/13). Click here for more details.
The MSBL will have several presentations at the upcoming BMES 2013 Annual Meeting in Seattle from September 25-28, 2013. First, congrats to Anthony Recidoro, who will present his poster “Neuronal Regulation of Fin Regeneration in Zebrafish” on Saturday from 9:30AM to 1:00PM. Also, on Thursday Ron will give a talk for his abstract entitled “The Skeleton As A Complex System: Emergent Bone Loss Signatures Following Neuromuscular Injury In Zebrafish” (“Animal Models in Musculoskeletal Diseases”, 8:00AM to 9:30AM).
Congrats to Anthony Recidoro for getting his abstract accepted for a combined plenary poster and oral session at the 2013 ASBMR conference in Baltimore! The details of the talk/poster are listed below:
Abstract Title: Neuroskeletal Signaling In The Regenerating Zebrafish Fin: Remote Inhibition of Osteoblast Function via Disruption of Cholinergic Signaling
Oral Session: 10/04/13, 4:15 PM – 5:45 PM, Room 307-310
Poster Session: 10/04/13, 5:45 PM – 7:00 PM, Discovery Hall-Hall C
We are starting to present some of our zebrafish findings at various conferences and the initial feedback has been great. We were recently awarded two plenary session presentations, one of which was a talk at the 8th European Zebrafish Meeting in Barcelona (the other is a plenary poster and oral session presentation at the upcoming ASBMR Meeting which I will be posting about later). The title of the talk was “Systems-Based Discovery in the Zebrafish Skeleton Using Micro-Computed Tomography”.
It was a fantastic experience going to my first zebrafish meeting. There were so many great talks given, with my personal favorite being an invited lecture by Nobel laureate Sydney Brenner. It was incredibly inspiring to be given a personal account of his discoveries and to hear his vision for the future of biology and genetics.
Joyce was there as well and snapped this picture during the presentation:
Had a great time at the ASME SBC Conference in Sun River, Oregon. The abstract we presented entitled “Fluid Dynamic Gauging-Based Assay for High-Throughput Investigation of Cellular Mechanotransduction” can be seen by clicking here.
Great news! “Introduction to Cell Mechanics and Mechanobiology”just received a fantastic review by Dr. Guy Genin in the latest edition of Cellular and Molecular Bioengineering. An excerpt is copied below (the full review can be seen by clicking here):
“When is a field ready for a new introductory text? One indication is that, despite a wide range of outstanding advanced texts available, many of us find ourselves drawing from a great number of these texts when introducing a subject to graduate students. This is certainly the case for me when teaching graduate students about cell mechanics and mechanobiology. But more telling is the fact that one of the field’s flagship journals has not until now published its first book review. The new text from Jacobs, Huang, and Kwon is fully worthy of the honor of being the first text reviewed in Cellular and Molecular Bioengineering. After reading through the clear, simple, but rigorous text, I can say that their work does far more than just tie together some important notes in a single binding. I will argue here that this text is potentially transformative for the field, much in the way that the famous texts by Beer and Johnston in the 1960s were transformative for the undergraduate study of mechanics of materials and machines.”
Congrats to Anthony for getting his abstract accepted to the UW Undergraduate Research Symposium on Friday, May 17th 2013. Look for his poster entitled “Neuronal Regulation of Regenerative Bone Growth in Zebrafish” in Mary Gates Hall 241 at Easel #168 between 12:45 and 2:15.
Great news! Ron has been awarded a New Faculty Research Fund Grant from the UW Department of Orthopaedics entitled “Biophysical Control of Bone Regeneration”. We are exploring the role of biophysical stimuli in controlling regenerative processes. The figure on the right shows a zebrafish tail fin prior to (“A”), immediately following (“B”), and 14 days post amputation (“C”). In this project, we seek to assess the ability to use biophysical stimuli to control regeneration in the zebrafish fin.
It has been a busy few months but I finally got around to publishing a post introducing some of our new lab members: Amanda Roof, Anthony Recidoro, and Jamil Qazi. Please visit the lab website to learn more about them.
We are currently seeking an undergraduate student with MATLAB and/or other computer programming experience to assist with a research project (6-12 hours a week, 10 hours on average) focused on high-throughput analysis of MicroCT data in zebrafish.
Slowly but surely, we are building up the lab. This weekend, we took a road trip down to Oregon to pick up a used zebrafish housing rack from the Goldfish Garage. The rack is a six shelf stand alone system from Aquaneering. This system makes housing our fish much easier and dramatically increases our capacity, letting us house several thousands of fish.
It was definitely a great time, and worth the trip to see the fascinating collection of exotic fish!
I am happy to announce that Introduction to Cell Mechanics and Mechanobiology has been published! This is a project that has been almost five years in the making. The impetus for the book started from a course on cell mechanics that myself, Chris Jacobs, and Ellen Kuhl taught at Stanford University. While teaching the course, we realized that there was a need for a self-contained text that could teach cell mechanics to students of diverse academic backgrounds, both in terms of level (senior undergraduate to graduate students) and disciplines (biology, various disciplines of engineering, physics, etc.).
Our solution was to write a book with the goal of using cell mechanics and mechanobiology as a substrate to teach introductory biology and mechanics. The first half of the book focuses on fundamentals, giving introductions to cell/molecular biology as well as the basic analytical tools of solid mechanics, fluid mechanics, experimental mechanics, and statistical mechanics. The second half focuses on practices, giving examples in which mechanics can be used to analyze cellular structures as well as better understand cell function and behavior.
We hope that it will be a useful resource for those already in the field and will help to attract and train new investigators in this very exciting discipline.
Bone adaptation to mechanical loading has long been hypothesized to be mediated by alterations in the flow of skeletal fluid (either blood or interstitial fluid, the plasma-like fluid which leaks out from blood vessels). In general, visualizing skeletal fluid flow in a living organism is difficult, as the bone is mineralized and surrounded by soft tissue. To quantify skeletal fluid flow in mice, we have previously had to rely on a technique which combined confocal microscopy, FRAP (e.g., see this paper and this paper), and computational modeling (e.g., see this paper) to back-calculate flow velocities from FRAP measurements.
A favorable attribute about zebrafish is that measuring skeletal fluid flow (either interstitial fluid or blood) is much easier compared to mice due to their optical transparency. For example, below is a movie we made of an adult zebrafish caudal fin, which is transparent. The rectangular objects are interconnected segments of bony fin rays, and between them you can see the red blood cells traversing through blood vessels running through and around the bones.
Below is the same field of view imaged using epi-fluorescence. In this case, we labeled the bone using a fluorescent dye called calcein, which preferentially binds to calcium ions on the bone surface. The ability to simultaneously visualize blood and interstitial fluid flow and bone growth in zebrafish is extremely powerful and will allow us to gain new insights into the role of skeletal fluid flow in regulating bone health.
Cool cover! The field of mechanobiology has grown so quickly in the last few decades…but who would’ve thought that Science would publish a special issue on the topic? That’s right, a Special Issue dedicated to forces in development. There are a number of extremely cool papers in there, I highly recommend checking it out.
Woo hoo! Our textbook, Introduction to Cell Mechanics and Mechanobiology, is now available for pre-order on amazon.com. This is a project which my co-authors and I have worked on for over four years, and I am extremely excited for it to be introduced to the public (slated for Winter 2012). Anybody interested in learning about the fundamentals of cell mechanics and mechanobiology in a self-contained format may want to give it a look. More details to follow!
Welcome to my research blog! I hope to use it as forum to talk about a wide variety of topics including cool advancements in musculoskeletal, systems, and/or mechanobiology, and other random topics that I think others might find interesting. Thanks for visiting!